(Targeted Vectors A.K.A Gene targeting (also, replacement strategy based on homologous recombination)
If you already thought it was bad out there, times that fuckery by a million and i assure you it's even worse than that, they are genome specific & aerosolized and sprayed down on us, put in food and water supplies just to make sure, and actively target any genome that they have a sample of and want to aim at. (Targeted Vectors Gene targeting (also, replacement strategy based on homologous recombination) is a genetic technique that uses homologous recombination to modify an endogenous gene. ... Gene targeting requires the creation of a specific vector for each gene of interest.), so anyone that used those ancestry dna sites are screwed for sure. Transfer of the Genetic Material to the Cells
Although, systemic intravenous route can be applied to deliver the genetic material to the cells, local delivery methods are more commonly used such as surgical, percutaneous, US and computed tomography guided and by means of catheters.
The viral vectors can be divided into two types: Integrating and non-integrating viral vectors. The former, such as, retroviral, lentiviral, and adeno-associated viral vectors, can integrate into the human genome; whereas the non-integrating vector (e.g., adenoviralvector) is maintained in the nucleus without integrating into the chromosomal DNA, so that the transgene is apt to lose during cell division and expression of the foreign gene is transient. In a packaging cell, the essential components for further propagating of viruses can be provided in trans, which enable the viral vectors to be packaged as the viral particles and to deliver genes to the targeted cells. Certainly, this is a dead-end infection, because the vectors lack the essential components for viruses’ propagation. Recombinant viral vectors can lead to the generation of infectious parental viruses. This is a principle frequently used in viral vector design in gene therapy.[4]
TELEGRAPH.CO.UK
World must prepare for biological weapons that target ethnic groups based on genetics, says Cambridge UniversityBiological weapons could be built which target individuals in a specific ethnic group based on their DNA, a report by the University of Cambridge has warned.
Article in Expert Opinion on Drug Delivery 12(6):1-15 · November 2014 with 70 Reads
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Abstract
Introduction: Lung cancer has the highest mortality rate among all cancers in both men and women. Aerosol delivery is a noninvasive method for gene delivery to the lungs, although efficient and biocompatible vectors need to be developed for lung cancer therapy. Areas covered: This review summarizes recent advances in airway gene delivery for lung cancer treatment in animal models using viral vectors or cationic polymers. Viral vectors including lentiviruses and adenoviruses have been used for airway gene delivery because of their high transfection efficiency. Cationic polymers have also been developed for aerosol gene therapy owing to their biocompatibility and ease of modification. Expert opinion: Efficient delivery and specific promoters are needed for lung cancer therapy. Capsid engineering or PEGylation can lower immunogenicity. Moreover, immunotherapy and oncolytic viruses need to be tested with aerosol delivery for lung cancer therapy. Meanwhile, naturally existing cationic materials may allow the development of novel and biocompatible carriers. In combination with various technologies for aerosol delivery, novel and specific carriers could be developed for lung cancer therapy in the future. Finally, standardized protocols for quantifying and manufacturing viral vectors and cationic polymers need to be developed in order to ensure biosafety.
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References (102)
... The genetic material carried by retroviruses can be transmitted to daughter cells when the cell divides. ( Hong et al., 2015). Despite these advantages, retroviruses can insert their cargo randomly into the host genome, giving rise to the potential for insertional mutagenesis. ...
Chapter
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... Moreover, its positive charge facilitates the fusion with the negatively charged nuclear envelope phospholipids which in turn enhances transfection. However, the efficiency of PEI is faced with its cytotoxicity which is dependent on its molecular weight and configuration [33][34][35][36]. PEI-based polyplexes were synthesized for the aerosol delivery of interleukin 12 (IL-12) gene for the treatment of osteosarcoma lung metastases. ...
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... (18) In this regard, we have previously used several non viral or viral vectors to transport DNA or siRNA or shRNA through aerosol delivery for lung cancer treatment. (19) For example, we delivered shAkt1 by glycerol triacrylate-spermine (GT- SPE) to suppress lung cancer progression. (20) Similarly, we used polyspermine to deliver the Pdcd4 gene that greatly reduced tumor size in K-ras LA1 lung cancer model mice. ...
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... The major problems of chemotherapy are toxicity, lack of specificity and drug resistance [98]. In recent years, pulmonary delivery of RNAi molecules is being explored as a new treatment for lung cancer [99]. Genes that are associated with tumor cell growth and drug resistance are potential targets. ...
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... Aerosol delivery enables non-invasive and efficient delivery of macromolecules to the lung [19, 20]. Moreover, aerosol delivery using various cationic carriers and viral vectors has been successfully used in lung cancer therapy [21]. We previously developed a GPT-SPE cationic polymer which showed biocompatibility and high transfection efficiency [22]. ...
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... Applications of RNAi-based experiments to in vivo cancer targeting and therapy experiments using synthetic siRNAs also have been proven as effective methods [12][13][14]. Various in vivo experiments using cationic carriers with DNA have been performed for cancer gene therapy [15] . For example, cancer gene therapy using biocompatible poly(amino ester) based on glycerol propoxylate triacrylate spermine (GPT-SPE) for delivering siRNA targeting OPN (siOPN) was performed. ...
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... Dr. Bhumika Aggarwal and his group compare the efficacy and tolerability with salmeterol and fluticasone given individually, and with other fixed-dose combinations namely formoterol/fluticasone, formoterol/beclometasone and formoterol/ mometasone furoate, all delivered via pMDI [7]. Dr. Myung-Haing Cho and his collaborators summarize recent advances in airway gene delivery for lung cancer treatment in animal models using viral vectors or cationic polymers [8]. Viral vectors including lentiviruses and adenoviruses have been used for airway gene delivery because of their high transfection efficiency. ...
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