Shared from Sophie Murishwar 😁😁😁😉🌍☮️
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Breaking news:
the UK government finally admit the PCR test cannot detect an infectious virus.
😲😲😲 Page 6.
Bet you won't hear this on the news. Published by Public Health England on the 28th of October 2020. Let's be the media for truth and share this info. 🙂
How can they justify anything now?🤷♂️
Government link below
page 6 of the document reads as follows
Understanding cycle threshold (Ct) in SARS-CoV-2 RT-PCR
6
Figure 2. Genome of SARS-CoV-2 with the most common RT-PCR targets
highlighted
👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇RT-PCR detects presence of viral genetic material in a sample but is not able to distinguish whether infectious virus is present.
The quantity of intact virus in upper respiratory swabs will be affected by factors that are endogenous and exogenous to laboratory methods.
Laboratory exogenous factors
1. The adequacy of sample collection.
2. The quantity of virus at the collection site.
3. The presence of inhibitors.
Laboratory endogenous factors
1. The total volume of sample collection buffer/medium.
2. The sample preparation method (heat, lysis methods).
3. The laboratory reagent volumes used in each step of the RT-PCR process.
4. The RT-PCR assay of choice.
What is a Ct value?
The cycle threshold (Ct) can be defined as the thermal cycle number at which the
fluorescent signal exceeds that of the background and thus passes the threshold for
positivity (Figure 1, page 5).
A typical RT-PCR assay will have a maximum of 40 thermal cycles. The lower the Ct
value the higher the quantity of viral genetic material in the sample (as an approximate
proxy for viral load). Ct values obtained in this way are semi-quantitative and are able to
distinguish between high and low viral load. A 3-point increase in Ct value is roughly
equivalent to a 10-fold decrease in the quantity of viral genetic material.
In some circumstances, Ct values can be used as a more quantitative technique to
accurately measure the number of viral copies per cell in the original sample – however,
this requires that the sample is tested alongside verified standard dilutions and there is
fixed sample input alongside quantification of cellular content of a swab sample(1,2).
Most diagnostic laboratories do not routinely perform this quantification for respiratory
viral swabs – quantitative PCR is more common in measuring blood-borne viral load.
